Cytological Effects of Alcoholic Extract Cnidoscolus aconitifolius on Bone Marrow Aspirate in Adult Male Wistar Rats

Abstract

This study was designed at investigating the effect of alcoholic extract of Cnidoscolus aconitifolius on bone marrow aspirate of adult male Wistar rats. The rats were acclimatized for two weeks, weighed and sorted into four groups (A - D) of three animals each with corresponding weights in the same group. Alcoholic extract of Cnidoscolus aconitifolius was administered orally to each animal in groups A-D at the rate of 0ml/kg, 200ml/kg, 300mI/kg and 500ml/kg respectively. Group A was the control group and was served with feed and water liberally. Subsequently, the animals were sacrificed and the bone marrow aspirate harvested from the medullary canal of the femur and fixed in 95% alcohol. Staining was with Papanicolaou stain. Results showed a dose dependent distortion in aspirate morphology with decrease in aspirate cellularity. Generally, the result showed a marked dose dependent distortion of bone marrow cytology with replacement of marrow with fibrous and reduced cellularity.