Effects of Non-Selective Cyclooxygenase Inhibitors-Ibuprofen and Paracetamol on Glomerulogenesis in Sprague Dawley Rats

Abstract

The effect of non-selective cyclooxygenase inhibitors Ibuprofen and Paracetamol on glomerulogenesis in Sprague-Dawley rats was studied. Fifteen female rats weighing between 165g-179g were randomly divided into three groups (A, B, and C) of five rats each. Group A animals received distilled water orally and served as control. The ibuprofen treated rats (group B) received doses of 3.5mg/ kg/day while paracetamol treated rats (group C) received doses of 7.3mg/kg/day respectively by gavage. The animals were allowed feed and water liberally. Drug administration commenced from 10^th day of gestation to 13^th day after parturition. The neonatal rats were then sacrificed for tissue processing. Histopathology results showed ibuprofen induced derangement in the neonatal kidneys. The manifestation include highly shrunken glomeruli, fibrosis, interstitial nephritis, hyaline droplets in the tubules, hemorrhage and infiltrated cells while paracetamol treated group showed lesser effects on the kidney architecture such as marked reduction in size of glomerulus (hypoplasia) and apparently wide capsular spaces some of which lacked glomeruli, shrunken glomeruli with hemorrhage, hyaline droplets and hyperchromatic cells. Both findings reflect renal function impairment.