The Significance of Adaptive Changes in Rat Haemoglobin Microenvironment and Electophoretic Fractions during Barocamera Hypoxia

Abstract

The haemoglobin heterogenous system and some aspects of the microenvironment of the respiratory protein were investigated in albino rats, trained to high altitude hypoxia in barocamera conditions. Hypoxia was simulated in a hypoxic cage at a “height” of 6000m above sea level. Electrophoretic analysis of haemoglobin, the oxyhaemoglobin dissociation curve and the activity of the 2, 3-diphosphoglycerate bypass were studied in the 1^st, 3^rd 5^th and 10^th days of hypoxic exposure. While decrease in the concentration of fractions located towards the cathode was recorded, an additional 7^th fraction appeared at the anode during the adaptation process. Decrease in the metabolic activity of 2, 3-diphosphoglycerate mutase with corresponding decrease in concentration of 2, 3-diphosphglycerate (2, 3-DPG) which correlates with leftward shift of the oxyhaemoglobin dissociation curve was observed in the 3^rd and 5^th days of the experiment. A tendency towards normalization of the haemoglobin P₅₀, concentrated of 2, 3-DPG and activity of 2, 3-DPG mutase was recorded in the 10^th day of the exercise.